Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome

• Main Authors: Dziekan, Jerzy Michal, Wirjanata, Grennady, Dai, Lingyun, Go, Ka Diam, Yu, Han, Lim, Yan Ting, Chen, Liyan, Wang, Loo Chien, Puspita, Brenda, Prabhu, Nayana, Sobota, Radoslaw M., Nordlund, Pär, Bozdech, Zbynek
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/155221
• Institution: Nanyang Technological University

Despite decades of research, little is known about the cellular targets and the mode of action of the vast majority of antimalarial drugs. We recently demonstrated that the cellular thermal shift assay (CETSA) protocol in its two variants: the melt curve and the isothermal dose-response, represents...