A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides

A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides

Joining peptides and oligonucleotides offers potential benefits, but current methods remain laborious. Here we present a novel approach towards enzymatic ligation of the two modalities through the development of tag phosphoramidites that readily couple onto oligonucleotides. This simple and highly e...

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Main Authors: Tan, Derrick Jing Yang, Cheong, Vee Vee, Lim, Kah Wai, Phan, Anh Tuân
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2022
Subjects:
Science::Biological sciences
Science::Chemistry
Peptides
Oligonucleotides
Online Access:https://hdl.handle.net/10356/158325
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Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-158325
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spelling sg-ntu-dr.10356-1583252023-02-28T20:03:19Z A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides Tan, Derrick Jing Yang Cheong, Vee Vee Lim, Kah Wai Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Biological sciences Science::Chemistry Peptides Oligonucleotides Joining peptides and oligonucleotides offers potential benefits, but current methods remain laborious. Here we present a novel approach towards enzymatic ligation of the two modalities through the development of tag phosphoramidites that readily couple onto oligonucleotides. This simple and highly efficient approach paves the way towards streamlined development and production of peptide/protein-oligonucleotide conjugates. Nanyang Technological University National Research Foundation (NRF) Submitted/Accepted version This research was supported by Singapore National Research Foundation (NRF-NRFI2017-09) and Nanyang Technological University. 2022-05-18T01:53:06Z 2022-05-18T01:53:06Z 2021 Journal Article Tan, D. J. Y., Cheong, V. V., Lim, K. W. & Phan, A. T. (2021). A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides. Chemical Communications, 57(45), 5507-5510. https://dx.doi.org/10.1039/d1cc01348c 1359-7345 https://hdl.handle.net/10356/158325 10.1039/d1cc01348c 45 57 5507 5510 en NRF-NRFI2017-09 Chemical Communications © 2021 The Royal Society of Chemistry. All rights reserved. This paper was published in Chemical Communications and is made available with permission of The Royal Society of Chemistry. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Science::Chemistry
Peptides
Oligonucleotides
spellingShingle Science::Biological sciences
Science::Chemistry
Peptides
Oligonucleotides
Tan, Derrick Jing Yang
Cheong, Vee Vee
Lim, Kah Wai
Phan, Anh Tuân
A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
description Joining peptides and oligonucleotides offers potential benefits, but current methods remain laborious. Here we present a novel approach towards enzymatic ligation of the two modalities through the development of tag phosphoramidites that readily couple onto oligonucleotides. This simple and highly efficient approach paves the way towards streamlined development and production of peptide/protein-oligonucleotide conjugates.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Tan, Derrick Jing Yang
Cheong, Vee Vee
Lim, Kah Wai
Phan, Anh Tuân
format Article
author Tan, Derrick Jing Yang
Cheong, Vee Vee
Lim, Kah Wai
Phan, Anh Tuân
author_sort Tan, Derrick Jing Yang
title A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
title_short A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
title_full A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
title_fullStr A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
title_full_unstemmed A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
title_sort modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
publishDate 2022
url https://hdl.handle.net/10356/158325
_version_ 1759857394270076928

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