pH-controlled protein orthogonal ligation using asparaginyl peptide ligases

• Main Authors: Zhang, Dingpeng, Wang, Zhen, Hu, Side, Balamkundu, Seetharamsing, To, Janet, Zhang, Xiaohong, Lescar, Julien, Tam, James P., Liu, Chuan-Fa
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2022
• 主題: Science::Biological sciences; Peptide Asparaginyl Ligase; Biocatalysis
• 在線閱讀: https://hdl.handle.net/10356/159343
• 機構: Nanyang Technological University

Peptide asparaginyl ligases (PALs) catalyze transpeptidation at the Asn residue of a short Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn substrates at around neutral pH, PALs have been used extensively for peptide ligation at asparaginyl junctions. PALs also...