Human PERK rescues unfolded protein response-deficient yeast cells

• Main Authors: Yap, Wei Sheng, Thibault, Guillaume
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; Unfolded Protein Response; Endoplasmic Reticulum Stress; IRE1; PERK; Synthetic Biology; Yeast Human
• Online Access: https://hdl.handle.net/10356/161984
• Institution: Nanyang Technological University

Protein folding and quality control is tightly regulated at the endoplasmic reticulum (ER), and its disruption is associated with many diseases. In eukaryotes, the accumulation of unfolded protein in the ER is sensed by the three sensors, IRE1, PERK, and ATF6 to activate the unfolded protein response (UPR) to restore ER homeostasis. However, uncoupling the sensing of each sensor and their respective downstream pathways has been challenging as the absence of one is compensated by the remaining two sensors. Here, we report a fully functional human PERK (hPERK) chimeric protein expressed in Saccharomyces cerevisiae that could be used for high throughput screen to identify new PERK inhibitory or activating compounds as well as to characterize the PERK stress sensing mechanisms.