CRISPR/Cas9-induced DNA damage enriches for mutations in a p53-linked interactome: implications for CRISPR-based therapies

• Main Authors: Jiang, Long, Ingelshed, Katrine, Shen, Yunbing, Boddul, Sanjaykumar V., Iyer, Vaishnavi Srinivasan, Kasza, Zsolt, Sedimbi, Saikiran, Lane, David P., Wermeling, Fredrik
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; Gene-Therapy; Cancer
• Online Access: https://hdl.handle.net/10356/163561
• Institution: Nanyang Technological University

Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient in p53 and in genes of a core CRISPR-p53 tumor suppressor interactome. Such enrichment could predispose to cancer developm...