Characterisation of splicing regulation by PRPF40A/B paralogues

Alternative splicing plays an essential role in various physiological processes such as cellular differentiation and development. A tight regulation of alternative splicing is critical for the maintenance of normal physiological processes and splicing dysregulation has been found to be implicated in...

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Main Author: Koh, Jace
Other Authors: Francesc Xavier Roca Castella
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/167213
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1672132023-05-29T15:33:17Z Characterisation of splicing regulation by PRPF40A/B paralogues Koh, Jace Francesc Xavier Roca Castella School of Biological Sciences xroca@ntu.edu.sg Science::Biological sciences Alternative splicing plays an essential role in various physiological processes such as cellular differentiation and development. A tight regulation of alternative splicing is critical for the maintenance of normal physiological processes and splicing dysregulation has been found to be implicated in various diseases. As splicing factors play important roles in the regulation of alternative splicing, this project seeks to understand the functional differences and similarities between the splicing factor paralogues PRPF40A and PRPF40B in human promyelocytic leukaemia (HL-60) cells. Preliminary data has shown PRPF40A to be critical for HL-60 cell survival as PRPF40A knockdown led to significant levels of cell death, which could be rescued by PRPF40B overexpression. In this study, transcriptomic analysis revealed that PRPF40A and PRPF40B regulate exons with differing properties. While PRPF40A regulates exons flanked by short high GC content introns, PRPF40B regulates exons flanked by introns of lower GC content. Additionally, the region of highest divergence between both paralogues does not seem to play a role in functionally differentiating PRPF40A and PRPF40B. Overall, both paralogues do not fully overlap in their splicing regulatory function, and this may potentially be implicated in myeloid cell physiology. Bachelor of Science in Biological Sciences 2023-05-24T13:09:21Z 2023-05-24T13:09:21Z 2023 Final Year Project (FYP) Koh, J. (2023). Characterisation of splicing regulation by PRPF40A/B paralogues. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/167213 https://hdl.handle.net/10356/167213 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Koh, Jace
Characterisation of splicing regulation by PRPF40A/B paralogues
description Alternative splicing plays an essential role in various physiological processes such as cellular differentiation and development. A tight regulation of alternative splicing is critical for the maintenance of normal physiological processes and splicing dysregulation has been found to be implicated in various diseases. As splicing factors play important roles in the regulation of alternative splicing, this project seeks to understand the functional differences and similarities between the splicing factor paralogues PRPF40A and PRPF40B in human promyelocytic leukaemia (HL-60) cells. Preliminary data has shown PRPF40A to be critical for HL-60 cell survival as PRPF40A knockdown led to significant levels of cell death, which could be rescued by PRPF40B overexpression. In this study, transcriptomic analysis revealed that PRPF40A and PRPF40B regulate exons with differing properties. While PRPF40A regulates exons flanked by short high GC content introns, PRPF40B regulates exons flanked by introns of lower GC content. Additionally, the region of highest divergence between both paralogues does not seem to play a role in functionally differentiating PRPF40A and PRPF40B. Overall, both paralogues do not fully overlap in their splicing regulatory function, and this may potentially be implicated in myeloid cell physiology.
author2 Francesc Xavier Roca Castella
author_facet Francesc Xavier Roca Castella
Koh, Jace
format Final Year Project
author Koh, Jace
author_sort Koh, Jace
title Characterisation of splicing regulation by PRPF40A/B paralogues
title_short Characterisation of splicing regulation by PRPF40A/B paralogues
title_full Characterisation of splicing regulation by PRPF40A/B paralogues
title_fullStr Characterisation of splicing regulation by PRPF40A/B paralogues
title_full_unstemmed Characterisation of splicing regulation by PRPF40A/B paralogues
title_sort characterisation of splicing regulation by prpf40a/b paralogues
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/167213
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