Expanding the substrate specificity of peptide asparaginyl ligase

• Main Author: Chua, Niying
• Other Authors: Julien Lescar
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2023
• Subjects: Science::Biological sciences::Evolution; Science::Biological sciences::Biochemistry
• Online Access: https://hdl.handle.net/10356/168598
• Institution: Nanyang Technological University

Through amide bond formation, peptide ligases (such as sortase A and subtiligase) introduce specific protein modifications, providing a specific and affordable tool for protein conjugation. Although sortases and subtiligases are capable of site-specific protein conjugations, their slow ligation rea...