A cascade enzymatic reaction scheme for irreversible transpeptidative protein ligation

• Main Authors: Xia, Yiyin, Li, Fupeng, Zhang, Xiaohong, Balamkundu, Seetharamsing, Tang, Fan, Hu, Side, Lescar, Julien, Tam, James P., Liu, Chuan-Fa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Enzymes; Molar Ratio
• Online Access: https://hdl.handle.net/10356/169065
• Institution: Nanyang Technological University

Enzymatic peptide ligation holds great promise in the study of protein functions and development of protein therapeutics. Owing to their high catalytic efficiency and a minimal tripeptide recognition motif, peptidyl asparaginyl ligases (PALs) are particularly useful tools for bioconjugation. However...