Atomic insights of an up and down conformation of the Acinetobacter baumannii F₁-ATPase subunit ε and deciphering the residues critical for ATP hydrolysis inhibition and ATP synthesis

• Main Authors: Saw, Wuan Geok, Le, Khoa Cong Minh, Shin, Joon, Kwek, Jes Hui Min, Wong, Chui Fann, Ragunathan, Priya, Fong, Tuck Choy, Müller, Volker, Grüber, Gerhard
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; ATP Synthesis; Bioenergetics
• Online Access: https://hdl.handle.net/10356/170125
• Institution: Nanyang Technological University

The Acinetobacter baumannii F1 FO -ATP synthase (α3 :β3 :γ:δ:ε:a:b2 :c10 ), which is essential for this strictly respiratory opportunistic human pathogen, is incapable of ATP-driven proton translocation due to its latent ATPase activity. Here, we generated and purified the first recombinant A. bauma...