KOPI: kinase inhibitor proteome impact analysis

KOPI: kinase inhibitor proteome impact analysis

Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI'...

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Bibliographic Details
Main Authors: Li, Ginny Xiaohe, Zhao, Tianyun, Wang, Loo Chien, Choi, Hyungwon, Lim, Yan Ting, Sobota, Radoslaw M.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
Subjects:
Science::Biological sciences
Antineoplastic Agents
Phosphorylation
Online Access:https://hdl.handle.net/10356/171280
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Institution: Nanyang Technological University
Language: English
Description
Summary:Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI's utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine's non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.

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