KOPI: kinase inhibitor proteome impact analysis

Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI'...

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Main Authors:	Li, Ginny Xiaohe, Zhao, Tianyun, Wang, Loo Chien, Choi, Hyungwon, Lim, Yan Ting, Sobota, Radoslaw M.
Other Authors:	School of Biological Sciences
Format:	Article
Language:	English
Published:	2023
Subjects:	Science::Biological sciences Antineoplastic Agents Phosphorylation
Online Access:	https://hdl.handle.net/10356/171280
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Institution:	Nanyang Technological University
Language:	English

id	sg-ntu-dr.10356-171280
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spelling	sg-ntu-dr.10356-1712802023-10-23T15:32:12Z KOPI: kinase inhibitor proteome impact analysis Li, Ginny Xiaohe Zhao, Tianyun Wang, Loo Chien Choi, Hyungwon Lim, Yan Ting Sobota, Radoslaw M. School of Biological Sciences Institute of Molecular and Cell Biology, ASTAR Science::Biological sciences Antineoplastic Agents Phosphorylation Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI's utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine's non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation. Agency for Science, Technology and Research (ASTAR) National Research Foundation (NRF) Published version This research was supported by ASTAR Core funding, Young Investigator Grant 2015 (YIG-2015) awarded to R.M.S by the Biomedical Research Council of the Agency for Science, Technology and Research (ASTAR). R.M.S is also supported by Singapore National Research Foundation under its NRF-SIS (NRF2017_SISFP08) “SingMass” share infrastructure scheme. 2023-10-20T02:50:44Z 2023-10-20T02:50:44Z 2022 Journal Article Li, G. X., Zhao, T., Wang, L. C., Choi, H., Lim, Y. T. & Sobota, R. M. (2022). KOPI: kinase inhibitor proteome impact analysis. Scientific Reports, 12(1), 13015-. https://dx.doi.org/10.1038/s41598-022-16557-w 2045-2322 https://hdl.handle.net/10356/171280 10.1038/s41598-022-16557-w 35906361 2-s2.0-85135173483 1 12 13015 en Scientific Reports © 2022 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	Science::Biological sciences Antineoplastic Agents Phosphorylation
spellingShingle	Science::Biological sciences Antineoplastic Agents Phosphorylation Li, Ginny Xiaohe Zhao, Tianyun Wang, Loo Chien Choi, Hyungwon Lim, Yan Ting Sobota, Radoslaw M. KOPI: kinase inhibitor proteome impact analysis
description	Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI's utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine's non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.
author2	School of Biological Sciences
author_facet	School of Biological Sciences Li, Ginny Xiaohe Zhao, Tianyun Wang, Loo Chien Choi, Hyungwon Lim, Yan Ting Sobota, Radoslaw M.
format	Article
author	Li, Ginny Xiaohe Zhao, Tianyun Wang, Loo Chien Choi, Hyungwon Lim, Yan Ting Sobota, Radoslaw M.
author_sort	Li, Ginny Xiaohe
title	KOPI: kinase inhibitor proteome impact analysis
title_short	KOPI: kinase inhibitor proteome impact analysis
title_full	KOPI: kinase inhibitor proteome impact analysis
title_fullStr	KOPI: kinase inhibitor proteome impact analysis
title_full_unstemmed	KOPI: kinase inhibitor proteome impact analysis
title_sort	kopi: kinase inhibitor proteome impact analysis
publishDate	2023
url	https://hdl.handle.net/10356/171280
_version_	1781793737539584000

KOPI: kinase inhibitor proteome impact analysis

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