The study of G-quadruplex in supercoiled DNA

It is demonstrated that DNA gyrase, an essential bacterial enzyme which possesses a unique ability to introduce negative supercoils into DNA, can drive G-quadruplex generation from plasmid DNA under the intracellular concentrations of cations in prokaryotes. The outcomes of our investigations could...

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Main Author: Lv, Bei
Other Authors: Li Tianhu
Format: Theses and Dissertations
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/68928
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-689282023-02-28T23:47:57Z The study of G-quadruplex in supercoiled DNA Lv, Bei Li Tianhu School of Physical and Mathematical Sciences DRNTU::Science::Chemistry It is demonstrated that DNA gyrase, an essential bacterial enzyme which possesses a unique ability to introduce negative supercoils into DNA, can drive G-quadruplex generation from plasmid DNA under the intracellular concentrations of cations in prokaryotes. The outcomes of our investigations could suggest that prokaryotic cells might utilize this topological enzyme to regulate generation of a G-quadruplex to comply with their subsequent cellular functions. On the other hand, our in vitro studies also show that both breakdown of some thermodynamically stable non-B structures and resumption of B-conformation of DNA can take place during the courses of isothermal helicase-dependent amplification (HDA). We speculate that the pathway of making the non-B structures repairable is the relieving of accumulated torsional stress that is caused by the positive supercoiling. Our findings suggest that living organisms might have evolved this distinct and economical pathway for repairing their physically altered DNA structures. DOCTOR OF PHILOSOPHY (SPMS) 2016-08-10T07:18:29Z 2016-08-10T07:18:29Z 2016 Thesis Lv, B. (2016). The study of G-quadruplex in supercoiled DNA. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/68928 10.32657/10356/68928 en 134 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Chemistry
spellingShingle DRNTU::Science::Chemistry
Lv, Bei
The study of G-quadruplex in supercoiled DNA
description It is demonstrated that DNA gyrase, an essential bacterial enzyme which possesses a unique ability to introduce negative supercoils into DNA, can drive G-quadruplex generation from plasmid DNA under the intracellular concentrations of cations in prokaryotes. The outcomes of our investigations could suggest that prokaryotic cells might utilize this topological enzyme to regulate generation of a G-quadruplex to comply with their subsequent cellular functions. On the other hand, our in vitro studies also show that both breakdown of some thermodynamically stable non-B structures and resumption of B-conformation of DNA can take place during the courses of isothermal helicase-dependent amplification (HDA). We speculate that the pathway of making the non-B structures repairable is the relieving of accumulated torsional stress that is caused by the positive supercoiling. Our findings suggest that living organisms might have evolved this distinct and economical pathway for repairing their physically altered DNA structures.
author2 Li Tianhu
author_facet Li Tianhu
Lv, Bei
format Theses and Dissertations
author Lv, Bei
author_sort Lv, Bei
title The study of G-quadruplex in supercoiled DNA
title_short The study of G-quadruplex in supercoiled DNA
title_full The study of G-quadruplex in supercoiled DNA
title_fullStr The study of G-quadruplex in supercoiled DNA
title_full_unstemmed The study of G-quadruplex in supercoiled DNA
title_sort study of g-quadruplex in supercoiled dna
publishDate 2016
url https://hdl.handle.net/10356/68928
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