Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes...

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Bibliographic Details
Main Authors: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
Other Authors: Maas, Stefan
Format: Article
Language:English
Published: 2015
Subjects:
RNA and DNA FISH
Online Access:https://hdl.handle.net/10356/80218
http://hdl.handle.net/10220/38859
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Institution: Nanyang Technological University
Language: English
Description
Summary:Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and singlemolecule level in cellular contexts.

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