Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes...

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Main Authors: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
Other Authors: Maas, Stefan
Format: Article
Language:English
Published: 2015
Subjects:
RNA and DNA FISH
Online Access:https://hdl.handle.net/10356/80218
http://hdl.handle.net/10220/38859
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-802182023-02-28T16:59:55Z Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH Basu, Reelina Lai, Lan-Tian Meng, Zhenyu Wu, Jun Shao, Fangwei Zhang, Li-Feng Maas, Stefan School of Biological Sciences School of Physical and Mathematical Sciences RNA and DNA FISH Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and singlemolecule level in cellular contexts. NMRC (Natl Medical Research Council, S’pore) Published version 2015-11-20T03:20:24Z 2019-12-06T13:45:09Z 2015-11-20T03:20:24Z 2019-12-06T13:45:09Z 2014 Journal Article Basu, R., Lai, L. T., Meng, Z., Wu, J., Shao, F., & Zhang, L. F. (2014). Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. PLoS ONE, 9(9), e107425-. 1932-6203 https://hdl.handle.net/10356/80218 http://hdl.handle.net/10220/38859 10.1371/journal.pone.0107425 25226542 en PLoS ONE © 2014 Basu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 8 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic RNA and DNA FISH
spellingShingle RNA and DNA FISH
Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
description Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and singlemolecule level in cellular contexts.
author2 Maas, Stefan
author_facet Maas, Stefan
Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
format Article
author Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
author_sort Basu, Reelina
title Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
title_short Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
title_full Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
title_fullStr Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
title_full_unstemmed Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH
title_sort using amino-labeled nucleotide probes for simultaneous single molecule rna-dna fish
publishDate 2015
url https://hdl.handle.net/10356/80218
http://hdl.handle.net/10220/38859
_version_ 1759853854641356800

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