Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

• Main Authors: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
• Other Authors: Maas, Stefan
• Format: Article
• Language: English
• Published: 2015
• Subjects: RNA and DNA FISH
• Online Access: https://hdl.handle.net/10356/80218; http://hdl.handle.net/10220/38859
• Institution: Nanyang Technological University

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long noncoding RNAs being identified in the current research. With probes...