Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

• Main Authors: Low, Wei Ching, Rujitanaroj, Pim-On, Lee, Dong-Keun, Kuang, Jinghao, Messersmith, Phillip B., Chan, Jerry Kok Yen, Chew, Sing Yian
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2015
• Subjects: Electrospinning; Neuronal differentiation; RE-1 silencing transcription factor; Sustained release; Neural differentiation
• Online Access: https://hdl.handle.net/10356/80820; http://hdl.handle.net/10220/38902
• Institution: Nanyang Technological University

In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ϵ-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating f...