Klf2 Is an Essential Factor that Sustains Ground State Pluripotency

• Main Authors: Yeo, Jia-Chi, Jiang, Jianming, Tan, Zi-Ying, Yim, Guo-Rong, Ng, Jia-Hui, Göke, Jonathan, Kraus, Petra, Liang, Hongqing, Gonzales, Kevin Andrew Uy, Chong, Han-Chung, Tan, Cheng-Peow, Lim, Yee-Siang, Tan, Nguan-Soon, Lufkin, Thomas, Ng, Huck-Hui
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Online Access: https://hdl.handle.net/10356/82151; http://hdl.handle.net/10220/41099
• Institution: Nanyang Technological University

The maintenance of mouse embryonic stem cells (mESCs) requires LIF and serum. However, a pluripotent “ground state,” bearing resemblance to preimplantation mouse epiblasts, can be established through dual inhibition (2i) of both prodifferentiation Mek/Erk and Gsk3/Tcf3 pathways. While Gsk3 inhibitio...