Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles

Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone...

Full description

Saved in:
Bibliographic Details
Main Authors: Li, Yan, Wong, Ying Lei, Lee, Michelle Yueqi, Li, Qingxin, Wang, Qing-Yin, Lescar, Julien, Shi, Pei-Yong, Kang, CongBao
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2017
Subjects:
Dengue-Virus
Membranproteine
Online Access:https://hdl.handle.net/10356/83047
http://hdl.handle.net/10220/42374
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
Description
Summary:Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.

Similar Items