Butelase-mediated cyclization and ligation of peptides and proteins

• Main Authors: Qiu, Yibo, Nguyen, Giang Kien Truc, Cao, Yuan, Hemu, Xinya, Liu, Chuan-Fa, Tam, James Pingkwan
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Butelase; Cyclization
• Online Access: https://hdl.handle.net/10356/84599; http://hdl.handle.net/10220/41879
• Institution: Nanyang Technological University

Enzymes that catalyze efficient macrocyclization or site-specific ligation of peptides and proteins can enable tools for drug design and protein engineering. Here we describe a protocol to use butelase 1, a recently discovered peptide ligase, for high-efficiency cyclization and ligation of peptides...