NS3 helicase from dengue virus specifically recognizes viral RNA sequence to ensure optimal replication

• Main Authors: Swarbrick, Crystall M. D., Basavannacharya, Chandrakala, Chan, Kitti W. K., Chan, Shu-Ann, Singh, Daljit, Wei, Na, Phoo, Wint Wint, Luo, Dahai, Lescar, Julien, Vasudevan, Subhash G.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Dengue Virus; RNA Replication; DRNTU::Science::Biological sciences::Genetics
• Online Access: https://hdl.handle.net/10356/88387; http://hdl.handle.net/10220/45733
• Institution: Nanyang Technological University

The protein–RNA interactions within the flavivirus replication complex (RC) are not fully understood. Our structure of dengue virus NS3 adenosine triphosphatase (ATPase)/helicase bound to the conserved 5′ genomic RNA 5′-AGUUGUUAGUCU-3′ reveals that D290 and R538 make specific interactions with G2 an...