Structural insights into substrate recognition in proton-dependent oligopeptide transporters

Short-chain peptides are transported across membranes through promiscuous proton-dependent oligopeptide transporters (POTs)—a subfamily of the major facilitator superfamily (MFS). The human POTs, PEPT1 and PEPT2, are also involved in the absorption of various drugs in the gut as well as transport to...

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Main Authors: Quistgaard, Esben M., Jong, Agnes Jin Oi, Nordlund, Pär, Löw, Christian, Guettou, Fatma, Trésaugues, Lionel, Moberg, Per, Jegerschöld, Caroline, Zhu, Lin
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/99088
http://hdl.handle.net/10220/12615
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-990882023-02-28T17:04:43Z Structural insights into substrate recognition in proton-dependent oligopeptide transporters Quistgaard, Esben M. Jong, Agnes Jin Oi Nordlund, Pär Löw, Christian Guettou, Fatma Trésaugues, Lionel Moberg, Per Jegerschöld, Caroline Zhu, Lin School of Biological Sciences Short-chain peptides are transported across membranes through promiscuous proton-dependent oligopeptide transporters (POTs)—a subfamily of the major facilitator superfamily (MFS). The human POTs, PEPT1 and PEPT2, are also involved in the absorption of various drugs in the gut as well as transport to target cells. Here, we present a structure of an oligomeric POT transporter from Shewanella oneidensis (PepTSo2), which was crystallized in the inward open conformation in complex with the peptidomimetic alafosfalin. All ligand-binding residues are highly conserved and the structural insights presented here are therefore likely to also apply to human POTs. Published version 2013-07-31T06:19:19Z 2019-12-06T20:03:09Z 2013-07-31T06:19:19Z 2019-12-06T20:03:09Z 2013 2013 Journal Article Guettou, F., Quistgaard, E. M., Trésaugues, L., Moberg, P., Jegerschöld, C., Zhu, L., Jong, A. J. O., Nordlund, P.,& Löw, C. (2013). Structural insights into substrate recognition in proton-dependent oligopeptide transporters. EMBO reports,14(9), 749-845. https://hdl.handle.net/10356/99088 http://hdl.handle.net/10220/12615 10.1038/embor.2013.107 23867627 en EMBO reports © 2013 EUROPEAN MOLECULAR BIOLOGY ORGANIZATION. This paper was published in EMBO reports and is made available as an electronic reprint (preprint) with permission of EUROPEAN MOLECULAR BIOLOGY ORGANIZATION. The paper can be found at the following official DOI: http://dx.doi.org/10.1038/embor.2013.107. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description Short-chain peptides are transported across membranes through promiscuous proton-dependent oligopeptide transporters (POTs)—a subfamily of the major facilitator superfamily (MFS). The human POTs, PEPT1 and PEPT2, are also involved in the absorption of various drugs in the gut as well as transport to target cells. Here, we present a structure of an oligomeric POT transporter from Shewanella oneidensis (PepTSo2), which was crystallized in the inward open conformation in complex with the peptidomimetic alafosfalin. All ligand-binding residues are highly conserved and the structural insights presented here are therefore likely to also apply to human POTs.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Quistgaard, Esben M.
Jong, Agnes Jin Oi
Nordlund, Pär
Löw, Christian
Guettou, Fatma
Trésaugues, Lionel
Moberg, Per
Jegerschöld, Caroline
Zhu, Lin
format Article
author Quistgaard, Esben M.
Jong, Agnes Jin Oi
Nordlund, Pär
Löw, Christian
Guettou, Fatma
Trésaugues, Lionel
Moberg, Per
Jegerschöld, Caroline
Zhu, Lin
spellingShingle Quistgaard, Esben M.
Jong, Agnes Jin Oi
Nordlund, Pär
Löw, Christian
Guettou, Fatma
Trésaugues, Lionel
Moberg, Per
Jegerschöld, Caroline
Zhu, Lin
Structural insights into substrate recognition in proton-dependent oligopeptide transporters
author_sort Quistgaard, Esben M.
title Structural insights into substrate recognition in proton-dependent oligopeptide transporters
title_short Structural insights into substrate recognition in proton-dependent oligopeptide transporters
title_full Structural insights into substrate recognition in proton-dependent oligopeptide transporters
title_fullStr Structural insights into substrate recognition in proton-dependent oligopeptide transporters
title_full_unstemmed Structural insights into substrate recognition in proton-dependent oligopeptide transporters
title_sort structural insights into substrate recognition in proton-dependent oligopeptide transporters
publishDate 2013
url https://hdl.handle.net/10356/99088
http://hdl.handle.net/10220/12615
_version_ 1759856238756102144