Longitudinal single-cell RNA sequencing of patient-derived primary cells reveals drug-induced infidelity in stem cell hierarchy

• Main Authors: Sharma, A, Cao, E.Y, Kumar, V, Zhang, X, Leong, H.S, Wong, A.M.L, Ramakrishnan, N, Hakimullah, M, Teo, H.M.V, Chong, F.T, Chia, S, Thangavelu, M.T, Kwang, X.L, Gupta, R, Clark, J.R, Periyasamy, G, Iyer, N.G, DasGupta, R
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: Nature Publishing Group 2020
• Subjects: 4 (4 chlorophenyl) 2,3,9 trimethyl 6h thieno[3,2 f][1,2,4]triazolo[4,3 a][1,4]diazepine 6 acetic acid tert butyl ester; cisplatin; cytokeratin 18; cytokeratin 8; epithelial cell adhesion molecule; histone H3; RNA; transcription factor Sox2; transcription factor Sox9; uvomorulin; vimentin; antineoplastic agent; adaptation; animal experiment; animal model; Article; cancer chemotherapy; cancer resistance; cell plasticity; cell proliferation; chromatin; controlled study; head and neck squamous cell carcinoma; histone acetylation; human; human cell; mouse; nonhuman; phenotype; primary cell; RNA sequence; single cell analysis; stem cell; transcriptomics; tumor xenograft; animal; cancer stem cell; drug resistance; drug screening; gene expression profiling; gene expression regulation; genetic heterogeneity; genetics; knockout mouse; metabolism; mouth tumor; nonobese diabetic mouse; procedures; SCID mouse; sequence analysis; squamous cell carcinoma; tumor cell line; Animals; Antineoplastic Agents; Carcinoma, Squamous Cell; Cell Line, Tumor; Cisplatin; Drug Resistance, Neoplasm; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic Heterogeneity; Humans; Mice, Inbred NOD; Mice, Knockout; Mice, SCID; Mouth Neoplasms; Neoplastic Stem Cells; Sequence Analysis, RNA; Single-Cell Analysis; Xenograft Model Antitumor Assays
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/178380
• Institution: National University of Singapore

10.1038/s41467-018-07261-3