Development, optimization, and validation of novel anti-TEM1/CD248 affinity agent for optical imaging in cancer

• Main Authors: Li, C, Wang, J, Hu, J, Feng, Y, Hasegawa, K, Peng, X, Duan, X, Zhao, A, Mikitsh, J.L, Muzykantov, V.R, Chacko, A.-M, Pryma, D.A, Dunn, S.M, Coukos, G
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2020
• Subjects: endosialin; hybrid protein; single chain fragment variable antibody; tracer; tumor marker; CD248 protein, human; immunoglobulin fragment; leukocyte antigen; tumor antigen; animal cell; animal experiment; animal model; Article; binding affinity; breast cancer; cancer graft; controlled study; enzyme linked immunosorbent assay; fluorescence imaging; immunoreactivity; in vitro study; in vivo study; isotope labeling; lymph node metastasis; mouse; near infrared imaging system; neoplasm; nonhuman; ovary cancer; patient selection; positron emission tomography; process optimization; protein purification; protein stability; radioactivity; single photon emission computer tomography; thermostability; tomography; tumor vascularization; validation process; Western blotting; animal; chemistry; disease model; genetic transfection; genetics; human; immunology; nude mouse; tissue distribution; tumor cell line; xenograft; Animals; Antigens, CD; Antigens, Neoplasm; Cell Line, Tumor; Disease Models, Animal; Heterografts; Humans; Immunoglobulin Fragments; Mice; Mice, Nude; Neoplasms; Optical Imaging; Recombinant Fusion Proteins; Tissue Distribution; Transfection
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/181778
• Institution: National University of Singapore

10.18632/oncotarget.2188