A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12

A novel functional beverage was produced from red seaweed (Pom-nang seaweed), by fermentation using a probiotic bacterium Lactobacillus plantarum DW12 as a starter culture. In addition to the normal fermentation products the bacterium produced γ-aminobutyric acid (GABA) and prebiotic compounds. A ce...

Full description

Saved in:
Bibliographic Details
Main Authors: Hayisama-ae W., Kantachote D., Bhongsuwan D., Nokkaew U., Chaiyasut C.
Format: Article
Language:English
Published: Universiti Putra Malaysia 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84907029430&partnerID=40&md5=331e07ae373db6712a48af490176e610
http://cmuir.cmu.ac.th/handle/6653943832/37632
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-37632
record_format dspace
spelling th-cmuir.6653943832-376322014-12-09T05:54:18Z A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12 Hayisama-ae W. Kantachote D. Bhongsuwan D. Nokkaew U. Chaiyasut C. A novel functional beverage was produced from red seaweed (Pom-nang seaweed), by fermentation using a probiotic bacterium Lactobacillus plantarum DW12 as a starter culture. In addition to the normal fermentation products the bacterium produced γ-aminobutyric acid (GABA) and prebiotic compounds. A cell free culture supernatant also inhibited the growth of potential foodborne pathogens and spoilage bacteria (Bacillus cereus TISTR2687, Staphylococcus aureus PSSCMI0004, Escherichia coli PSSCMI0001, Salmonella Typhi ATCC19430 and Vibrio parahaemolyticus PSSCMI0004). In addition, the cell free culture supernatant had antioxidant activity as shown by its ability to scavenge 2, 2/-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) at a level of 60 and 68%, respectively. Using in vitro tests this bacterium was resistant to 1 mM H2O2 for 8 h and grew at various pH values from 2 to 9 and survived for 12 h in the presence of bile salts (0.15-0.30%). Results of antibiotic susceptibility tests showed that 8 of 15 antibiotics were inhibitory to strain DW12 at susceptible and intermediate levels. The fermented red seaweed beverage (FSB) contained 5.41 log CFU/ml lactic acid bacteria, 1284 mg/L GABA, 11.79 mg/ml sugars (fructose, glucose and sucrose) and 0.31 mg/ml of fructooligosaccharides (FOS, kestore and fructosylnystose). Freeze dried FSB at the concentrations tested (0.5-2.0%), that contained 78.61-314.42 mg/100 ml of sugars and 2.10-8.20 mg FOS/100 ml acted as a prebiotic by promoting the growth of strain DW12. 2014-12-09T05:54:18Z 2014-12-09T05:54:18Z 2014 Article 19854668 http://www.scopus.com/inward/record.url?eid=2-s2.0-84907029430&partnerID=40&md5=331e07ae373db6712a48af490176e610 http://cmuir.cmu.ac.th/handle/6653943832/37632 English Universiti Putra Malaysia
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A novel functional beverage was produced from red seaweed (Pom-nang seaweed), by fermentation using a probiotic bacterium Lactobacillus plantarum DW12 as a starter culture. In addition to the normal fermentation products the bacterium produced γ-aminobutyric acid (GABA) and prebiotic compounds. A cell free culture supernatant also inhibited the growth of potential foodborne pathogens and spoilage bacteria (Bacillus cereus TISTR2687, Staphylococcus aureus PSSCMI0004, Escherichia coli PSSCMI0001, Salmonella Typhi ATCC19430 and Vibrio parahaemolyticus PSSCMI0004). In addition, the cell free culture supernatant had antioxidant activity as shown by its ability to scavenge 2, 2/-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) at a level of 60 and 68%, respectively. Using in vitro tests this bacterium was resistant to 1 mM H2O2 for 8 h and grew at various pH values from 2 to 9 and survived for 12 h in the presence of bile salts (0.15-0.30%). Results of antibiotic susceptibility tests showed that 8 of 15 antibiotics were inhibitory to strain DW12 at susceptible and intermediate levels. The fermented red seaweed beverage (FSB) contained 5.41 log CFU/ml lactic acid bacteria, 1284 mg/L GABA, 11.79 mg/ml sugars (fructose, glucose and sucrose) and 0.31 mg/ml of fructooligosaccharides (FOS, kestore and fructosylnystose). Freeze dried FSB at the concentrations tested (0.5-2.0%), that contained 78.61-314.42 mg/100 ml of sugars and 2.10-8.20 mg FOS/100 ml acted as a prebiotic by promoting the growth of strain DW12.
format Article
author Hayisama-ae W.
Kantachote D.
Bhongsuwan D.
Nokkaew U.
Chaiyasut C.
spellingShingle Hayisama-ae W.
Kantachote D.
Bhongsuwan D.
Nokkaew U.
Chaiyasut C.
A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
author_facet Hayisama-ae W.
Kantachote D.
Bhongsuwan D.
Nokkaew U.
Chaiyasut C.
author_sort Hayisama-ae W.
title A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
title_short A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
title_full A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
title_fullStr A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
title_full_unstemmed A potential synbiotic beverage from fermented red seaweed (Gracilaria fisheri) using Lactobacillus plantarum DW12
title_sort potential synbiotic beverage from fermented red seaweed (gracilaria fisheri) using lactobacillus plantarum dw12
publisher Universiti Putra Malaysia
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84907029430&partnerID=40&md5=331e07ae373db6712a48af490176e610
http://cmuir.cmu.ac.th/handle/6653943832/37632
_version_ 1681421382105169920