Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean

© 2015, Journal of Pure and Applied Microbiology. All rights reserved. This study aimed to select the strain of Bacillus spp. as a starter culture for enriching isoflavone aglycones in traditional Thai fermented soybean (thua-nao) production. Forty-one strains of Bacillus spp. from Thai thua-nao and...

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Main Authors: Rewat Phongphisutthinant, Pairote Wiriyacharee, Sakda Preunglampoo, Posri Leelapat, Pattama Kanjanakeereetumrong, Saisamorn Lamyong
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/44506
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spelling th-cmuir.6653943832-445062018-04-25T07:51:24Z Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean Rewat Phongphisutthinant Pairote Wiriyacharee Sakda Preunglampoo Posri Leelapat Pattama Kanjanakeereetumrong Saisamorn Lamyong Agricultural and Biological Sciences © 2015, Journal of Pure and Applied Microbiology. All rights reserved. This study aimed to select the strain of Bacillus spp. as a starter culture for enriching isoflavone aglycones in traditional Thai fermented soybean (thua-nao) production. Forty-one strains of Bacillus spp. from Thai thua-nao and Japanese natto were characterized and divided into 5 groups; Bacillus licheniformis, Bacillus coagulans, Bacillus subtilis, Bacillus pumilus and Bacillus megaterium. Each strain was used to produce thua-nao. As a result, Bacillus PR03 produced the highest amount of isoflavone aglycones (146 mg/100g dry weight; 3.81 times higher than the amount in soybean) and was selected as the starter culture. According to 16S rDNA sequencing, Bacillus PR03 was identified as B. coagulans with 99.93% identical to IAM12463 strain. B. coagulans have never been reported as predominant bacteria in soybean fermentation. Therefore, this is the first research using B. coagulans as predominant bacteria for producing thua-nao. To enrich isoflavone aglycones in thua-nao production by B. coagulans PR03, it was found that the fermentation time of 5 d resulted in the optimal contents of daidzein, genistein, glycitein and total aglycones; 94.16, 68.29, 6.33 and 168.77 mg/100 g dry weight, respectively. With improved functional properties, the enriched isoflavone aglycones thua-nao could be applied as an ingredient in functional food development. 2018-01-24T04:43:53Z 2018-01-24T04:43:53Z 2015-01-01 Journal 09737510 2-s2.0-85037066433 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037066433&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/44506
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Rewat Phongphisutthinant
Pairote Wiriyacharee
Sakda Preunglampoo
Posri Leelapat
Pattama Kanjanakeereetumrong
Saisamorn Lamyong
Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
description © 2015, Journal of Pure and Applied Microbiology. All rights reserved. This study aimed to select the strain of Bacillus spp. as a starter culture for enriching isoflavone aglycones in traditional Thai fermented soybean (thua-nao) production. Forty-one strains of Bacillus spp. from Thai thua-nao and Japanese natto were characterized and divided into 5 groups; Bacillus licheniformis, Bacillus coagulans, Bacillus subtilis, Bacillus pumilus and Bacillus megaterium. Each strain was used to produce thua-nao. As a result, Bacillus PR03 produced the highest amount of isoflavone aglycones (146 mg/100g dry weight; 3.81 times higher than the amount in soybean) and was selected as the starter culture. According to 16S rDNA sequencing, Bacillus PR03 was identified as B. coagulans with 99.93% identical to IAM12463 strain. B. coagulans have never been reported as predominant bacteria in soybean fermentation. Therefore, this is the first research using B. coagulans as predominant bacteria for producing thua-nao. To enrich isoflavone aglycones in thua-nao production by B. coagulans PR03, it was found that the fermentation time of 5 d resulted in the optimal contents of daidzein, genistein, glycitein and total aglycones; 94.16, 68.29, 6.33 and 168.77 mg/100 g dry weight, respectively. With improved functional properties, the enriched isoflavone aglycones thua-nao could be applied as an ingredient in functional food development.
format Journal
author Rewat Phongphisutthinant
Pairote Wiriyacharee
Sakda Preunglampoo
Posri Leelapat
Pattama Kanjanakeereetumrong
Saisamorn Lamyong
author_facet Rewat Phongphisutthinant
Pairote Wiriyacharee
Sakda Preunglampoo
Posri Leelapat
Pattama Kanjanakeereetumrong
Saisamorn Lamyong
author_sort Rewat Phongphisutthinant
title Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
title_short Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
title_full Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
title_fullStr Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
title_full_unstemmed Selection of Bacillus spp. for isoflavone aglycones enriched Thua-nao, a traditional Thai fermented soybean
title_sort selection of bacillus spp. for isoflavone aglycones enriched thua-nao, a traditional thai fermented soybean
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037066433&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/44506
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