Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch
© 2017, Springer Science+Business Media New York. An amylolytic lactic acid bacterium isolate K-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as Entercoccus faecium based on 16S rRNA gene sequence analysis. An extracellular α-amylase was purified to homo...
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th-cmuir.6653943832-463562018-04-25T07:21:01Z Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch Kridsada Unban Apinun Kanpiengjai Goro Takata Keiko Uechi Wen Chien Lee Chartchai Khanongnuch Chemical Engineering Immunology and Microbiology Agricultural and Biological Sciences © 2017, Springer Science+Business Media New York. An amylolytic lactic acid bacterium isolate K-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as Entercoccus faecium based on 16S rRNA gene sequence analysis. An extracellular α-amylase was purified to homogeneity and the molecular weight of the purified enzyme was approximately 112 kDa with optimal pH value and temperature measured of 7.0 and 40 °C, respectively. It was stable at a pH range of 6.0–7.0, but was markedly sensitive to high temperatures and low pH conditions, even at a pH value of 5. Ba 2+ , Al 3+ , and Co 2+ activated enzyme activity. This bacterium was capable of producing 99.2% high optically pure L-lactic acid of 4.3 and 8.2 g/L under uncontrolled and controlled pH at 6.5 conditions, respectively, in the MRS broth containing 10 g/L cassava starch as the sole carbon source when cultivated at 37 °C for 48 h. A control pH condition of 6.5 improved and stabilized the yield of L-lactic acid production directly from starch even at a high concentration of starch at up to 150 g/L. This paper is the first report describing the properties of purified α-amylase from E. faecium. Additionally, pullulanase and cyclodextrinase activities were also firstly recorded from E. faecium K-1. 2018-04-25T06:53:41Z 2018-04-25T06:53:41Z 2017-09-01 Journal 15590291 02732289 2-s2.0-85013821707 10.1007/s12010-017-2436-1 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85013821707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46356 |
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Chemical Engineering Immunology and Microbiology Agricultural and Biological Sciences Kridsada Unban Apinun Kanpiengjai Goro Takata Keiko Uechi Wen Chien Lee Chartchai Khanongnuch Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
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© 2017, Springer Science+Business Media New York. An amylolytic lactic acid bacterium isolate K-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as Entercoccus faecium based on 16S rRNA gene sequence analysis. An extracellular α-amylase was purified to homogeneity and the molecular weight of the purified enzyme was approximately 112 kDa with optimal pH value and temperature measured of 7.0 and 40 °C, respectively. It was stable at a pH range of 6.0–7.0, but was markedly sensitive to high temperatures and low pH conditions, even at a pH value of 5. Ba 2+ , Al 3+ , and Co 2+ activated enzyme activity. This bacterium was capable of producing 99.2% high optically pure L-lactic acid of 4.3 and 8.2 g/L under uncontrolled and controlled pH at 6.5 conditions, respectively, in the MRS broth containing 10 g/L cassava starch as the sole carbon source when cultivated at 37 °C for 48 h. A control pH condition of 6.5 improved and stabilized the yield of L-lactic acid production directly from starch even at a high concentration of starch at up to 150 g/L. This paper is the first report describing the properties of purified α-amylase from E. faecium. Additionally, pullulanase and cyclodextrinase activities were also firstly recorded from E. faecium K-1. |
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Kridsada Unban Apinun Kanpiengjai Goro Takata Keiko Uechi Wen Chien Lee Chartchai Khanongnuch |
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Kridsada Unban Apinun Kanpiengjai Goro Takata Keiko Uechi Wen Chien Lee Chartchai Khanongnuch |
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Kridsada Unban |
title |
Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
title_short |
Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
title_full |
Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
title_fullStr |
Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
title_full_unstemmed |
Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch |
title_sort |
amylolytic enzymes acquired from l-lactic acid producing enterococcus faecium k-1 and improvement of direct lactic acid production from cassava starch |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85013821707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46356 |
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