Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. To valorize starchy waste from rice noodle factory, bioconversion of gelatinized starchy waste (GSW) to value-added product as L(+)-lactic acid, the monomer for polylactate synthesis, was investigated using amylolytic lactic acid...

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Main Authors: Kridsada Unban, Ramita Khanongnuch, Apinun Kanpiengjai, Kalidas Shetty, Chartchai Khanongnuch
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/70166
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spelling th-cmuir.6653943832-701662020-10-14T08:37:00Z Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1 Kridsada Unban Ramita Khanongnuch Apinun Kanpiengjai Kalidas Shetty Chartchai Khanongnuch Biochemistry, Genetics and Molecular Biology Chemical Engineering Immunology and Microbiology © 2020, Springer Science+Business Media, LLC, part of Springer Nature. To valorize starchy waste from rice noodle factory, bioconversion of gelatinized starchy waste (GSW) to value-added product as L(+)-lactic acid, the monomer for polylactate synthesis, was investigated using amylolytic lactic acid bacterium, Enterococcus faecium K-1. Screening for appropriate nitrogen source to replace expensive organic nitrogen sources revealed that corn steep liquor (CSL) was the most suitable regarding high efficacy for L(+)-LA achievement and low-cost property. The successful applying statistic experimental design, Plackett-Burman design incorporated with central composite design (CCD), predicted the maximum L(+)-LA of 93.07 g/L from the optimized medium (OM) containing 125.7 g/L GSW and 207.3 g/L CSL supplemented with CH3COONa, MgSO4, MnSO4, K2HPO4, CaCl2, (NH4)2HC6H5O7, and Tween80. Minimizing the medium cost by removal of all inorganic salts and Tween80 from OM was not an effect on L(+)-LA yield. Fermentation using the optimized medium without minerals (OM-Mi) containing only GSW (125.7 g/L) and CSL (207.3 g/L) in a 10-L fermenter was also successful. Thinning GSW with α-amylase from Lactobacillus plantarum S21 increased L(+)-LA productivity in the early stage of 24-h fermentation. Not only showing the feasible bioconversion process for GSW utilizing as a substrate for L(+)-LA production, this research also demonstrated the efficient model for industrial starchy waste valorization. 2020-10-14T08:25:07Z 2020-10-14T08:25:07Z 2020-10-01 Journal 15590291 02732289 2-s2.0-85084316077 10.1007/s12010-020-03314-w https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084316077&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70166
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
Kridsada Unban
Ramita Khanongnuch
Apinun Kanpiengjai
Kalidas Shetty
Chartchai Khanongnuch
Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
description © 2020, Springer Science+Business Media, LLC, part of Springer Nature. To valorize starchy waste from rice noodle factory, bioconversion of gelatinized starchy waste (GSW) to value-added product as L(+)-lactic acid, the monomer for polylactate synthesis, was investigated using amylolytic lactic acid bacterium, Enterococcus faecium K-1. Screening for appropriate nitrogen source to replace expensive organic nitrogen sources revealed that corn steep liquor (CSL) was the most suitable regarding high efficacy for L(+)-LA achievement and low-cost property. The successful applying statistic experimental design, Plackett-Burman design incorporated with central composite design (CCD), predicted the maximum L(+)-LA of 93.07 g/L from the optimized medium (OM) containing 125.7 g/L GSW and 207.3 g/L CSL supplemented with CH3COONa, MgSO4, MnSO4, K2HPO4, CaCl2, (NH4)2HC6H5O7, and Tween80. Minimizing the medium cost by removal of all inorganic salts and Tween80 from OM was not an effect on L(+)-LA yield. Fermentation using the optimized medium without minerals (OM-Mi) containing only GSW (125.7 g/L) and CSL (207.3 g/L) in a 10-L fermenter was also successful. Thinning GSW with α-amylase from Lactobacillus plantarum S21 increased L(+)-LA productivity in the early stage of 24-h fermentation. Not only showing the feasible bioconversion process for GSW utilizing as a substrate for L(+)-LA production, this research also demonstrated the efficient model for industrial starchy waste valorization.
format Journal
author Kridsada Unban
Ramita Khanongnuch
Apinun Kanpiengjai
Kalidas Shetty
Chartchai Khanongnuch
author_facet Kridsada Unban
Ramita Khanongnuch
Apinun Kanpiengjai
Kalidas Shetty
Chartchai Khanongnuch
author_sort Kridsada Unban
title Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
title_short Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
title_full Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
title_fullStr Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
title_full_unstemmed Utilizing Gelatinized Starchy Waste from Rice Noodle Factory as Substrate for L(+)-Lactic Acid Production by Amylolytic Lactic Acid Bacterium Enterococcus faecium K-1
title_sort utilizing gelatinized starchy waste from rice noodle factory as substrate for l(+)-lactic acid production by amylolytic lactic acid bacterium enterococcus faecium k-1
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084316077&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70166
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