Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells
Nickel, a heavy metal found in electronic wastes and fume from electronic cigarettes, induces neuronal cell death and is associated with neurocognitive impairment. Astrocytes are the first line of defense against nickel after entering the brain; however, the effects of nickel on astrocytes remain un...
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th-mahidol.763022022-08-04T15:55:30Z Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells Ruedeemars Yubolphan Suttinee Phuagkhaopong Kant Sangpairoj Nathawut Sibmooh Christopher Power Pornpun Vivithanaporn University of Alberta, Faculty of Medicine and Dentistry Faculty of Medicine Ramathibodi Hospital, Mahidol University Faculty of Medicine, Thammasat University Mahidol University Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Nickel, a heavy metal found in electronic wastes and fume from electronic cigarettes, induces neuronal cell death and is associated with neurocognitive impairment. Astrocytes are the first line of defense against nickel after entering the brain; however, the effects of nickel on astrocytes remain unknown. Herein, we investigated the effect of nickel exposure on cell survival and proliferation and the underlying mechanisms in U-87 MG human astrocytoma cells and primary human astrocytes. Intracellular nickel levels were elevated in U-87 MG cells in a dose- and time-dependent manner after exposure to nickel chloride. The median toxic concentrations of nickel in astrocytoma cells and primary human astrocytes were 600.60 and >1000 µM at 48 h post-exposure, respectively. Nickel exposure triggered apoptosis in concomitant with the decreased expression of anti-apoptotic B-cell lymphoma protein (Bcl-2) and increased caspase-3/7 activity. Nickel induced reactive oxygen species formation. Additionally, nickel suppressed astrocyte proliferation in a dose- and time-dependent manner by delaying G2 to M phase transition through the upregulation of cyclin B1 and p27 protein expression. These results indicate that nickel-induced cytotoxicity of astrocytes is mediated by the activation of apoptotic pathway and disruption of cell cycle regulation. 2022-08-04T08:12:45Z 2022-08-04T08:12:45Z 2021-01-16 Article Metallomics : integrated biometal science. Vol.13, No.1 (2021) 10.1093/mtomcs/mfaa006 1756591X 2-s2.0-85101469556 https://repository.li.mahidol.ac.th/handle/123456789/76302 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85101469556&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Ruedeemars Yubolphan Suttinee Phuagkhaopong Kant Sangpairoj Nathawut Sibmooh Christopher Power Pornpun Vivithanaporn Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
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Nickel, a heavy metal found in electronic wastes and fume from electronic cigarettes, induces neuronal cell death and is associated with neurocognitive impairment. Astrocytes are the first line of defense against nickel after entering the brain; however, the effects of nickel on astrocytes remain unknown. Herein, we investigated the effect of nickel exposure on cell survival and proliferation and the underlying mechanisms in U-87 MG human astrocytoma cells and primary human astrocytes. Intracellular nickel levels were elevated in U-87 MG cells in a dose- and time-dependent manner after exposure to nickel chloride. The median toxic concentrations of nickel in astrocytoma cells and primary human astrocytes were 600.60 and >1000 µM at 48 h post-exposure, respectively. Nickel exposure triggered apoptosis in concomitant with the decreased expression of anti-apoptotic B-cell lymphoma protein (Bcl-2) and increased caspase-3/7 activity. Nickel induced reactive oxygen species formation. Additionally, nickel suppressed astrocyte proliferation in a dose- and time-dependent manner by delaying G2 to M phase transition through the upregulation of cyclin B1 and p27 protein expression. These results indicate that nickel-induced cytotoxicity of astrocytes is mediated by the activation of apoptotic pathway and disruption of cell cycle regulation. |
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University of Alberta, Faculty of Medicine and Dentistry |
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University of Alberta, Faculty of Medicine and Dentistry Ruedeemars Yubolphan Suttinee Phuagkhaopong Kant Sangpairoj Nathawut Sibmooh Christopher Power Pornpun Vivithanaporn |
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Article |
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Ruedeemars Yubolphan Suttinee Phuagkhaopong Kant Sangpairoj Nathawut Sibmooh Christopher Power Pornpun Vivithanaporn |
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Ruedeemars Yubolphan |
title |
Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
title_short |
Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
title_full |
Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
title_fullStr |
Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
title_full_unstemmed |
Intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
title_sort |
intracellular nickel accumulation induces apoptosis and cell cycle arrest in human astrocytic cells |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/76302 |
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1763488662975152128 |