PENGARUH KONSENTRASI ASAM ASETAT DAN WAKTU EKSTRAKSI TERHADAP MUTU KOLAGEN LIMBAH KULIT IKAN NILA HITAM

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/29006/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12069
• Institution: Universitas Gadjah Mada

The objective of the research was studied the effect of acetic acid concentration and extraction time on the collagen quality of black tilapia leather. Black tilapia leather was processed into collagen as an alternative to increasing value-added of fisheries industry waste. Collagen of black tilapia was extracted by the treatment of acetic acid molarity, each: 0.25 M, 0.50 M, and 0.75 M (A factor) and extraction time of16 and 48 hours (Bfactor). Based on the analysis of variance is known that the interaction of those treatments (AB) didn't significantly effect on the yield (p>0.05). Collagen extraction of tilapia leather with 0.75 M of acetic acid at 16 hours, produces the greatest yield (5.97%), with denaturation temperature is 35.75°C, and quantitative composition of glipine, alanine, and glutamic amino acids were: 5395.82 ppm (52.99%),2979.15 ppm (22.08%), and 1684.42 ppm (7.45%). Based on the analysis of SDS-PAGE is known that the collagen contained were a component and f3 component, so that collagen of tilapia leather has type I collagen.