Phage display engineering of peptide substrates in enhancing ligation efficiency of peptide asparaginyl ligase

• Main Author: To, Weng Fui
• Other Authors: James P Tam
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2020
• Subjects: Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/143301
• Institution: Nanyang Technological University

OaAEP1b is a peptide asparaginyl ligase (PAL) that recognizes a C-terminal Asx-containing tripeptide motif (Asn/Asp-X-Y) to generate a site-specific Asn-X peptide bond with an incoming peptide. However, it shows weak peptide cyclase activity, as compared to other PALs, particularly butelase-1, the f...