Immobilized peptide asparaginyl ligases enhance stability and facilitate macrocyclization and site-specific ligation
The recently discovered peptide asparaginyl ligases (PALs) from cyclotide-producing plants are efficient and versatile tools for protein and peptide engineering. Here, we report immobilization of two glycosylated PALs, butelase-1 and VyPAL2, using three different attachment methods and their applica...
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| Main Authors: | , , , |
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| 其他作者: | |
| 格式: | Article |
| 語言: | English |
| 出版: |
2021
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| 主題: | |
| 在線閱讀: | https://hdl.handle.net/10356/148283 |
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| 機構: | Nanyang Technological University |
| 語言: | English |