Immobilized peptide asparaginyl ligases enhance stability and facilitate macrocyclization and site-specific ligation

• Main Authors: Hemu, Xinya, To, Janet, Zhang, Xiaohong, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science; Peptides; Covalent Couplings
• Online Access: https://hdl.handle.net/10356/148283
• Institution: Nanyang Technological University

The recently discovered peptide asparaginyl ligases (PALs) from cyclotide-producing plants are efficient and versatile tools for protein and peptide engineering. Here, we report immobilization of two glycosylated PALs, butelase-1 and VyPAL2, using three different attachment methods and their applica...