Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains

• Main Authors: Hemu, Xinya, Chan, Ning-Yu, Liew, Heng Tai, Hu, Side, Zhang, Xiaohong, Serra, Aida, Lescar, Julien, Liu, Chuan-Fa, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Pasparaginyl Ligase; Plant Legumain; Asparaginyl Endopeptidase; Butelase; Ligase-Activity Determinant
• Online Access: https://hdl.handle.net/10356/164031
• Institution: Nanyang Technological University

Peptide asparaginyl ligases (PALs) are Asn/Asp(Asx)-specific ligases that are useful for precision modifications of proteins and live-cell surfaces. PALs share high sequence and structural similarity to legumains, asparaginyl endopeptidases (AEPs) that primarily hydrolyze peptide bonds after Asx, th...