Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis

Cartilage degeneration poses a challenge to clinicians in spite of the various therapies that have been developed for its treatment. Cell-based therapy is promising due to its ability to restore the structure and function of the native cartilage as much as possible. Stromal-derived mesenchymal stem...

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Main Author: M. K. Mohamed Idris
Other Authors: Wang Dongan
Format: Final Year Project
Language:English
Published: 2011
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Online Access:http://hdl.handle.net/10356/45690
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-456902023-03-03T15:32:17Z Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis M. K. Mohamed Idris Wang Dongan School of Chemical and Biomedical Engineering DRNTU::Science::Biological sciences::Microbiology::Virology DRNTU::Science::Medicine::Tissue engineering Cartilage degeneration poses a challenge to clinicians in spite of the various therapies that have been developed for its treatment. Cell-based therapy is promising due to its ability to restore the structure and function of the native cartilage as much as possible. Stromal-derived mesenchymal stem cells (SMSCs) can be cultured to undergo chondrogenesis in-vitro. However, a serious drawback in this process is that the cells become hypertrophic and undergo apoptosis. We propose dosing the SMSCs with TGF-β3 to promote chondrogenesis. Simultaneously, since type I collagen (Col I) is inevitably elevated along the passages, it will undermine the mechanical strength of engineered cartilage. RNA interference (RNAi) would therefore be utilized to reduce its expression. To deliver both TGF-β3 and Col I-targeted small hairpin RNA (shRNA) to the cells, a variety of viral vectors would be tested, including adenoviral vectors and lentiviral vectors. Adenoviral vector induces a transient expression due to its episomal performance, whereas lentiviral vector leads to a more sustained expression since the vector can integrate its genome together with transgenes into the host genome. The results of this research suggest the promising potential of these viral vectors for the engineering of articular cartilage. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2011-06-16T03:45:24Z 2011-06-16T03:45:24Z 2011 2011 Final Year Project (FYP) http://hdl.handle.net/10356/45690 en Nanyang Technological University 81 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Virology
DRNTU::Science::Medicine::Tissue engineering
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Virology
DRNTU::Science::Medicine::Tissue engineering
M. K. Mohamed Idris
Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
description Cartilage degeneration poses a challenge to clinicians in spite of the various therapies that have been developed for its treatment. Cell-based therapy is promising due to its ability to restore the structure and function of the native cartilage as much as possible. Stromal-derived mesenchymal stem cells (SMSCs) can be cultured to undergo chondrogenesis in-vitro. However, a serious drawback in this process is that the cells become hypertrophic and undergo apoptosis. We propose dosing the SMSCs with TGF-β3 to promote chondrogenesis. Simultaneously, since type I collagen (Col I) is inevitably elevated along the passages, it will undermine the mechanical strength of engineered cartilage. RNA interference (RNAi) would therefore be utilized to reduce its expression. To deliver both TGF-β3 and Col I-targeted small hairpin RNA (shRNA) to the cells, a variety of viral vectors would be tested, including adenoviral vectors and lentiviral vectors. Adenoviral vector induces a transient expression due to its episomal performance, whereas lentiviral vector leads to a more sustained expression since the vector can integrate its genome together with transgenes into the host genome. The results of this research suggest the promising potential of these viral vectors for the engineering of articular cartilage.
author2 Wang Dongan
author_facet Wang Dongan
M. K. Mohamed Idris
format Final Year Project
author M. K. Mohamed Idris
author_sort M. K. Mohamed Idris
title Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
title_short Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
title_full Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
title_fullStr Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
title_full_unstemmed Co-delivery of TGF-β3 and COL I-targeting shRNA with adenoviral and lentiviral vectors for COL I-surpressed in vitro chondrogenesis
title_sort co-delivery of tgf-β3 and col i-targeting shrna with adenoviral and lentiviral vectors for col i-surpressed in vitro chondrogenesis
publishDate 2011
url http://hdl.handle.net/10356/45690
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