Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis

Crocodylus siamensis hemoglobin hydrolysates (CHHs) were obtained by trypsin and papain digestion at different incubation times (2, 4, 6 and 8 h) at 37 °C and subjected to antioxidant and anti-infl ammatory activity assessment. DPPH scavenging activity of CHH derived from trypsin was similar to inta...

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Main Authors: Jiraporn Lueangsakulthai, Nicholas Michael, Theeranan Temsiripong, Watcharee Khunkitti, Sompong Klaynongsruang, Nisachon Jangpromma
Language:English
Published: Science Faculty of Chiang Mai University 2019
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Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10250
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66920
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spelling th-cmuir.6653943832-669202019-12-03T06:32:24Z Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis Jiraporn Lueangsakulthai Nicholas Michael Theeranan Temsiripong Watcharee Khunkitti Sompong Klaynongsruang Nisachon Jangpromma hemoglobin hydrolysate papain hydrolysis trypsin hydrolysis anti-infl ammatory antioxidant Crocodylus siamensis hemoglobin hydrolysates (CHHs) were obtained by trypsin and papain digestion at different incubation times (2, 4, 6 and 8 h) at 37 °C and subjected to antioxidant and anti-infl ammatory activity assessment. DPPH scavenging activity of CHH derived from trypsin was similar to intact Hb. CHH derived from papain hydrolysis by 8-h hydrolysis (8h-CHHp) showed the highest DPPH scavenging activity at 56.86% antioxidant inhibition with IC50 value of 31 μg/ml. CHH derived from papain hydrolysis by 2-h hydrolysis (2h-CHHp) showed the highest reducing power activity at 0.99 mM Trolox equivalent and 4h-CHHt showed reducing activity at 0.16 mM Trolox equivalent (at concentration of 500 μg/ml). The linoleic peroxidation activity of CHH derived from papain hydrolysis by 6-h hydrolysis (6h-CHHp) and CHH derived from trypsin hydrolysis by 8-h hydrolysis (8h-CHHt) was increased in a dose-dependent manner with IC50 value of 5 μg/ml. The strongest anti-infl ammatory activity was found for 2h-CHHp, which displayed a high effi cacy in decreasing NO production of macrophage RAW 264.7 cells (46.86%) with no toxicity and signifi cantly reduced pro-infl ammatory cytokines interleukin-6 (IL-6) production to about 25.73 pg/ml. Taken collectively, the results of this work demonstrate that CHHs derived from papain hydrolysis possesses antioxidant and anti-infl ammatory activities, which provides support for the application against infl ammation and oxidative stress-related disorders. 2019-12-03T06:32:24Z 2019-12-03T06:32:24Z 2019 Chiang Mai Journal of Science 46, 5 (Sep 2019), 915 - 929 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10250 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66920 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
topic hemoglobin hydrolysate
papain hydrolysis
trypsin hydrolysis
anti-infl ammatory
antioxidant
spellingShingle hemoglobin hydrolysate
papain hydrolysis
trypsin hydrolysis
anti-infl ammatory
antioxidant
Jiraporn Lueangsakulthai
Nicholas Michael
Theeranan Temsiripong
Watcharee Khunkitti
Sompong Klaynongsruang
Nisachon Jangpromma
Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
description Crocodylus siamensis hemoglobin hydrolysates (CHHs) were obtained by trypsin and papain digestion at different incubation times (2, 4, 6 and 8 h) at 37 °C and subjected to antioxidant and anti-infl ammatory activity assessment. DPPH scavenging activity of CHH derived from trypsin was similar to intact Hb. CHH derived from papain hydrolysis by 8-h hydrolysis (8h-CHHp) showed the highest DPPH scavenging activity at 56.86% antioxidant inhibition with IC50 value of 31 μg/ml. CHH derived from papain hydrolysis by 2-h hydrolysis (2h-CHHp) showed the highest reducing power activity at 0.99 mM Trolox equivalent and 4h-CHHt showed reducing activity at 0.16 mM Trolox equivalent (at concentration of 500 μg/ml). The linoleic peroxidation activity of CHH derived from papain hydrolysis by 6-h hydrolysis (6h-CHHp) and CHH derived from trypsin hydrolysis by 8-h hydrolysis (8h-CHHt) was increased in a dose-dependent manner with IC50 value of 5 μg/ml. The strongest anti-infl ammatory activity was found for 2h-CHHp, which displayed a high effi cacy in decreasing NO production of macrophage RAW 264.7 cells (46.86%) with no toxicity and signifi cantly reduced pro-infl ammatory cytokines interleukin-6 (IL-6) production to about 25.73 pg/ml. Taken collectively, the results of this work demonstrate that CHHs derived from papain hydrolysis possesses antioxidant and anti-infl ammatory activities, which provides support for the application against infl ammation and oxidative stress-related disorders.
author Jiraporn Lueangsakulthai
Nicholas Michael
Theeranan Temsiripong
Watcharee Khunkitti
Sompong Klaynongsruang
Nisachon Jangpromma
author_facet Jiraporn Lueangsakulthai
Nicholas Michael
Theeranan Temsiripong
Watcharee Khunkitti
Sompong Klaynongsruang
Nisachon Jangpromma
author_sort Jiraporn Lueangsakulthai
title Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
title_short Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
title_full Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
title_fullStr Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
title_full_unstemmed Antioxidant and Anti-inflammatory Activities of the Siamese Crocodile (Crocodylus Siamensis) Hemoglobin Hydrolysate Derived from Trypsin and Papain Hydrolysis
title_sort antioxidant and anti-inflammatory activities of the siamese crocodile (crocodylus siamensis) hemoglobin hydrolysate derived from trypsin and papain hydrolysis
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10250
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66920
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