Expanding the ligation toolbox through protein engineering of peptide asparaginyl ligases (PALs)

• Main Author: Tan, Shaun Jun Hao
• Other Authors: James P Tam
• Format: Thesis-Master by Research
• Language: English
• Published: Nanyang Technological University 2023
• Subjects: Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/169289
• Institution: Nanyang Technological University

Asparaginyl endopeptidases (AEPs) are thiol proteases that cleave peptide bonds after Asn/Asp(Asx) residues. However, certain AEPs also make Asx bonds and which we named peptide asparaginyl ligases (PALs). PALs, ATP-independent and stand-alone, are ideal for site-specific modifications of proteins,...