Expanding the ligation toolbox through protein engineering of peptide asparaginyl ligases (PALs)
Asparaginyl endopeptidases (AEPs) are thiol proteases that cleave peptide bonds after Asn/Asp(Asx) residues. However, certain AEPs also make Asx bonds and which we named peptide asparaginyl ligases (PALs). PALs, ATP-independent and stand-alone, are ideal for site-specific modifications of proteins,...
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| Format: | Thesis-Master by Research |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/169289 |
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| Institution: | Nanyang Technological University |
| Language: | English |