Optimisation of over-expression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1

• Main Authors: Löw, Christian, Jegerschöld, Caroline, Kovermann, Michael, Moberg, Per, Nordlund, Pär
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Bacteria
• Online Access: https://hdl.handle.net/10356/95770; http://hdl.handle.net/10220/9349
• Institution: Nanyang Technological University

Progress in functional and structural studies of integral membrane proteins (IMPs) is lacking behind their soluble counterparts due to the great challenge in producing stable and homogeneous IMPs. Low natural abundance, toxicity when over-expressed and potential lipid requirements of IMPs are only a...