Optimisation of over-expression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1

• المؤلفون الرئيسيون: Löw, Christian, Jegerschöld, Caroline, Kovermann, Michael, Moberg, Per, Nordlund, Pär
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2013
• الموضوعات: DRNTU::Science::Biological sciences::Microbiology::Bacteria
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/95770; http://hdl.handle.net/10220/9349

Progress in functional and structural studies of integral membrane proteins (IMPs) is lacking behind their soluble counterparts due to the great challenge in producing stable and homogeneous IMPs. Low natural abundance, toxicity when over-expressed and potential lipid requirements of IMPs are only a...