Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments

© 2017 Elsevier Ltd The objectives of this study were to investigate the efficiency of three non-substrate specific enzymes on chitooligosaccharides (COS) production and to examine the antioxidant and antimicrobial properties of different average molecular weight (MW) COS. Two chitosans with 80 and...

Full description

Saved in:
Bibliographic Details
Main Authors: Thunnop Laokuldilok, Thipwimon Potivas, Nattapong Kanha, Suthat Surawang, Phisit Seesuriyachan, Sutee Wangtueai, Yuthana Phimolsiripol, Joe M. Regenstein
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017110756&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56507
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-56507
record_format dspace
spelling th-cmuir.6653943832-565072018-09-05T03:29:48Z Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments Thunnop Laokuldilok Thipwimon Potivas Nattapong Kanha Suthat Surawang Phisit Seesuriyachan Sutee Wangtueai Yuthana Phimolsiripol Joe M. Regenstein Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2017 Elsevier Ltd The objectives of this study were to investigate the efficiency of three non-substrate specific enzymes on chitooligosaccharides (COS) production and to examine the antioxidant and antimicrobial properties of different average molecular weight (MW) COS. Two chitosans with 80 and 90 degrees of deacetylation (DD) were hydrolyzed using lysozyme, papain, or cellulase (0.003% w/w) for 0–16 h. Papain showed the highest MW reduction of chitosan DD90. After 16 h of DD90 hydrolysis with papain, the average MW was 4.3 kDa which meets the requirement to be a COS (≤10 kDa). Materials produced from chitosan DD90 and papain with different MW of 5.1 (COS5), 14.3 (COS14), and 41.1 (COS41) kDa were used to determine antioxidant and antimicrobial activities. COS5 had the highest antioxidant activities of all of the assays used, i.e., the lowest 50% effective concentration (EC50) for DPPH radical scavenging activity, the highest reducing power, and the highest metal chelating activity. Escherichia coli, Salmonella typhimurium, and Salmonella enteritidis were most susceptible to COS5. All three COS were more effective against Escherichia coli than against the other pathogens. However, native chitosan inhibited Staphylococcus aureus, more efficiently. 2018-09-05T03:27:02Z 2018-09-05T03:27:02Z 2017-06-01 Journal 22124306 22124292 2-s2.0-85017110756 10.1016/j.fbio.2017.03.004 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017110756&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56507
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Thunnop Laokuldilok
Thipwimon Potivas
Nattapong Kanha
Suthat Surawang
Phisit Seesuriyachan
Sutee Wangtueai
Yuthana Phimolsiripol
Joe M. Regenstein
Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
description © 2017 Elsevier Ltd The objectives of this study were to investigate the efficiency of three non-substrate specific enzymes on chitooligosaccharides (COS) production and to examine the antioxidant and antimicrobial properties of different average molecular weight (MW) COS. Two chitosans with 80 and 90 degrees of deacetylation (DD) were hydrolyzed using lysozyme, papain, or cellulase (0.003% w/w) for 0–16 h. Papain showed the highest MW reduction of chitosan DD90. After 16 h of DD90 hydrolysis with papain, the average MW was 4.3 kDa which meets the requirement to be a COS (≤10 kDa). Materials produced from chitosan DD90 and papain with different MW of 5.1 (COS5), 14.3 (COS14), and 41.1 (COS41) kDa were used to determine antioxidant and antimicrobial activities. COS5 had the highest antioxidant activities of all of the assays used, i.e., the lowest 50% effective concentration (EC50) for DPPH radical scavenging activity, the highest reducing power, and the highest metal chelating activity. Escherichia coli, Salmonella typhimurium, and Salmonella enteritidis were most susceptible to COS5. All three COS were more effective against Escherichia coli than against the other pathogens. However, native chitosan inhibited Staphylococcus aureus, more efficiently.
format Journal
author Thunnop Laokuldilok
Thipwimon Potivas
Nattapong Kanha
Suthat Surawang
Phisit Seesuriyachan
Sutee Wangtueai
Yuthana Phimolsiripol
Joe M. Regenstein
author_facet Thunnop Laokuldilok
Thipwimon Potivas
Nattapong Kanha
Suthat Surawang
Phisit Seesuriyachan
Sutee Wangtueai
Yuthana Phimolsiripol
Joe M. Regenstein
author_sort Thunnop Laokuldilok
title Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
title_short Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
title_full Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
title_fullStr Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
title_full_unstemmed Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
title_sort physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017110756&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56507
_version_ 1681424704940802048