L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration
© 2020, Chiang Mai University. All rights reserved. The production efficacy of L-lactic acid production by Lactobacillus salivarius L105 was improved by statistical optimization. Plackett-Burman design was used to screen for the most influencing factors effect on L-lactic acid production. Among eigh...
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th-cmuir.6653943832-701792020-10-14T08:48:52Z L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration Nitaya Boontim Kridsada Unban Wasu Pathom-Aree Piyanuch Niamsup Chartchai Khanongnuch Saisamorn Lumyong Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2020, Chiang Mai University. All rights reserved. The production efficacy of L-lactic acid production by Lactobacillus salivarius L105 was improved by statistical optimization. Plackett-Burman design was used to screen for the most influencing factors effect on L-lactic acid production. Among eight nutritional parameters including glucose, beef extract, yeast extract, sodium acetate, trisodium phosphate, ammonium citrate, magnesium sulfate and manganese sulfate, yeast extract and trisodium phosphate were found to be the most significant effective factors (p<0.01) which were further optimized using central composite design (CCD) and response surface plot. A second order polynomial regression model estimated a maximal lactic acid production of 11.09 g/l whereby the levels of yeast extract and trisodium phosphate were 22.10 g and 11.09 g, respectively. The experimental value of 12.0 g/l lactic acid with the productivity of 0.63 g/l/h was achieved after cultivation in the optimized medium. Cultivation L. salivarious L105 using the optimized medium in 2.5-L jar fermenter under the controlled pH 6.5 at 37°C without aeration gave higher L-lactic acid approximately 1.5 times. L. salivarious L105 showed the osmotolerant characteristic at the high concentration of sugar up to 200 g/l. However, the improvement strategies i.e. medium composition, fermentation operation and bacterial strain improvement is necessary for achieving the higher productivity. 2020-10-14T08:25:15Z 2020-10-14T08:25:15Z 2020-09-01 Journal 01252526 2-s2.0-85090609860 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85090609860&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70179 |
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Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy Nitaya Boontim Kridsada Unban Wasu Pathom-Aree Piyanuch Niamsup Chartchai Khanongnuch Saisamorn Lumyong L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
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© 2020, Chiang Mai University. All rights reserved. The production efficacy of L-lactic acid production by Lactobacillus salivarius L105 was improved by statistical optimization. Plackett-Burman design was used to screen for the most influencing factors effect on L-lactic acid production. Among eight nutritional parameters including glucose, beef extract, yeast extract, sodium acetate, trisodium phosphate, ammonium citrate, magnesium sulfate and manganese sulfate, yeast extract and trisodium phosphate were found to be the most significant effective factors (p<0.01) which were further optimized using central composite design (CCD) and response surface plot. A second order polynomial regression model estimated a maximal lactic acid production of 11.09 g/l whereby the levels of yeast extract and trisodium phosphate were 22.10 g and 11.09 g, respectively. The experimental value of 12.0 g/l lactic acid with the productivity of 0.63 g/l/h was achieved after cultivation in the optimized medium. Cultivation L. salivarious L105 using the optimized medium in 2.5-L jar fermenter under the controlled pH 6.5 at 37°C without aeration gave higher L-lactic acid approximately 1.5 times. L. salivarious L105 showed the osmotolerant characteristic at the high concentration of sugar up to 200 g/l. However, the improvement strategies i.e. medium composition, fermentation operation and bacterial strain improvement is necessary for achieving the higher productivity. |
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Nitaya Boontim Kridsada Unban Wasu Pathom-Aree Piyanuch Niamsup Chartchai Khanongnuch Saisamorn Lumyong |
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Nitaya Boontim Kridsada Unban Wasu Pathom-Aree Piyanuch Niamsup Chartchai Khanongnuch Saisamorn Lumyong |
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Nitaya Boontim |
title |
L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
title_short |
L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
title_full |
L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
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L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
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L-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
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l-lactic acid production by lactobacillus salivarius l105 in optimized medium and effects of sugar concentration |
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2020 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85090609860&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70179 |
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