An inter-subunit protein-peptide interface that stabilizes the specific activity and oligomerization of the AAA+ chaperone Reptin

• Main Authors: Coufalova, Dominika, Remnant, Lucy, Hernychova, Lenka, Muller, Petr, Healy, Alan, Kannan, Srinivasaraghavan, Westwood, Nicholas, Verma, Chandra Shekhar, Vojtesek, Borek, Hupp, Ted R., Houston, Douglas R.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2020
• Subjects: Engineering::Bioengineering; Reptin; Protein
• Online Access: https://hdl.handle.net/10356/144645
• Institution: Nanyang Technological University

Reptin is a member of the AAA+ superfamily whose members can exist in equilibrium between monomeric apo forms and ligand bound hexamers. Inter-subunit protein-protein interfaces that stabilize Reptin in its oligomeric state are not well-defined. A self-peptide binding assay identified a protein-pept...