RNA secondary structure-based design of antisense peptide nucleic acids for modulating disease-associated aberrant tau pre-mRNA alternative splicing

• Main Authors: Ong, Alan Ann Lerk, Tan, Jiazi, Bhadra, Malini, Dezanet, Clément, Patil, Kiran M., Chong, Mei Sian, Kierzek, Ryszard, Decout, Jean-Luc, Roca, Xavier, Chen, Gang
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: RNA Structure; Strand Invasion; Science::Chemistry::Biochemistry
• Online Access: https://hdl.handle.net/10356/93533; http://hdl.handle.net/10220/49939
• Institution: Nanyang Technological University

Alternative splicing of tau pre-mRNA is regulated by a 5' splice site (5'ss) hairpin present at the exon 10–intron 10 junction. Single mutations within the hairpin sequence alter hairpin structural stability and/or the binding of splicing factors, resulting in disease-causing aberrant spli...