The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication

• Main Authors: Li X.-D., Shan C., Deng C.-L., Ye H.-Q., Shi P.-Y., Yuan Z.-M., Gong P., Zhang B.
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2019
• Subjects: complementary DNA; methyltransferase; nonstructural protein 5; RNA directed RNA polymerase; virus RNA; DNA directed RNA polymerase; NS5 protein, flavivirus; virus protein; animal cell; article; controlled study; electroporation; enzyme activity; Flavivirus infection; gene mutation; gene sequence; genetic transfection; immunofluorescence microscopy; immunofluorescence test; Japanese encephalitis virus; luciferase assay; molecular interaction; nonhuman; replicon; reverse transcription polymerase chain reaction; RNA extraction; RNA replication; RNA transcription; virus genome; virus mutant; virus plaque; virus replication; amino acid sequence; cell line; chemical phenomena; chemistry; Flavivirus; genetics; molecular genetics; nucleotide sequence; physiology; sequence alignment; Amino Acid Sequence; Cell Line; Conserved Sequence; DNA-Directed RNA Polymerases; Hydrophobic and Hydrophilic Interactions; Methyltransferases; Molecular Sequence Data; Sequence Alignment; Viral Nonstructural Proteins; Virus Replication
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161951
• Institution: National University of Singapore

10.1371/journal.pntd.0002891