Turning an asparaginyl endopeptidase into a peptide ligase

• Main Authors: Hemu, Xinya, El Sahili, Abbas, Hu, Side, Zhang, Xiaohong, Serra, Aida, Goh, Boon Chong, Darwis, Dina Amallia, Chen, Ming Wei, Sze, Siu Kwan, Liu, Chuan-Fa, Lescar, Julien, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science; Peptide Asparaginyl Ligase (PAL); Asparaginyl Endopeptidase (AEP)
• Online Access: https://hdl.handle.net/10356/148032
• Institution: Nanyang Technological University

Butelase-1 is a peptide asparaginyl ligase (PAL) that efficiently catalyzes peptide bond formation after Asn/Asp (Asx), whereas its homologue butelase-2 is an asparaginyl endopeptidase (AEP) that catalyzes the reverse reaction, hydrolyzing Asx-peptide bonds. Since PALs and AEPs share essentially sim...