Site-specific protein and cell surface engineering using asparaginyl peptide ligases

• Main Author: Zhang, DingPeng
• Other Authors: Liu Chuan Fa
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2022
• Subjects: Science::Biological sciences::Biochemistry
• Online Access: https://hdl.handle.net/10356/155535
• Institution: Nanyang Technological University

Peptidyl Asx-specific ligases (PALs) effect peptide ligation by catalyzing transpeptidation reactions at Asn/Asp-peptide bonds. Owing to their mild aqueous reaction conditions and high efficiency, PALs have emerged as powerful biotechnological tools for protein manipulation in recent years. PALs bel...